Composite

Part:BBa_K2217029:Design

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-25)


CMV Promoter + CMV enhancer + T7 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design aimed at generating Homology Directed Repair instead of Non-Homologous end Joining repair of Cas9 by knocking in the circular RNA as a Competing endogenous RNA enhancing its transcription to regulate miRNA action, so we designed Homology repair template (BBa_K2217009) to be transfected on a separate donor vector then terminated with CYC1 terminator(BBa_K2217008).

More information on the design of this part can be found at [http://2017.igem.org/Team:AFCM-Egypt/Design# Egypt-AFCM Design]

Source

Synthesized Fragment

References